Stable and sensitive SERS-based immunoassay enabled by core-shell immunoprobe and paper-based immunosubstrate
| Autor: | Guodong Wei, Hongmei Liu, Chenjie Gu, Tao Jiang, Ying Chen, Ziluo Su |
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| Rok vydání: | 2021 |
| Předmět: |
Detection limit
Materials science medicine.diagnostic_test Metals and Alloys Nanoparticle Nanotechnology engineering.material Condensed Matter Physics Dissociation (chemistry) Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention symbols.namesake law Immunoassay Materials Chemistry medicine symbols engineering Noble metal Electrical and Electronic Engineering Raman spectroscopy Instrumentation Raman scattering Chemiluminescence |
| Zdroj: | Sensors and Actuators B: Chemical. 347:130606 |
| ISSN: | 0925-4005 |
| Popis: | Aiming to augment the reliability and efficiency of surface enhanced Raman scattering (SERS)-active immunoprobe, sophisticated strategies that are able to avoid the dissociation of Raman molecules and noble metal nanoparticles (NPs) in practice have drawn a considerable attention. This study confirmed that Ag@polystyrene-block-poly(acrylic acid)@Ag core-shell nanostructures possessed superior stability compared with bare Ag NPs in phosphate buffer saline solution and human serum. Combined with a unique paper-based immunosubstrate with a satisfying enhancement factor of 9.71 × 105, a robust sandwich immunostructure was constructed and conducted to monitor carbohydrate antigen 19-9 (CA19-9), presenting a low limit of detection of about 10−4 U/mL. With these exceptional features, such a proposed protocol was finally utilized to detect CA19-9 in several clinical human sera, which shown much higher sensitivity than the traditional chemiluminescence analysis. |
| Databáze: | OpenAIRE |
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