| Autor: |
Yong VoonChen, Chong PeiPei, Choo KhiKhi, Ho SiongHock |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
British Microbiology Research Journal. 7:100-108 |
| ISSN: |
2231-0886 |
| DOI: |
10.9734/bmrj/2015/16464 |
| Popis: |
Aims: Cryptococcus neoformans is a fungal pathogen which infection caused devastating morbidity and mortality in immunocompromised patients. The present study investigated the effect of culture starting inoculum size and culture age towards cellular properties of C. neoformans and its interactions with mammalian host alveolar epithelial cells. Methodology: C. neoformans H99 was cultured at different starting inoculum sizes and collected at varied culture ages to examine the morphology of the yeast cells and agar invasion property. The interaction with host alveolar epithelial cells was assessed in vitro using A549 cells as the host cell model. Results: Visual observation demonstrated that cryptococci cultured with higher starting inoculum sizes and longer incubation periods displayed flocculation properties, aberrant morphologies with |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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