Immobilized penicillin G acylase with enhanced activity and stability using glutaraldehyde‐modified polydopamine‐coated Fe 3 O 4 nanoparticles
| Autor: | Zhenbin Chen, Zhangjun Chen, Chunli Liu, Boyuan Zhang, Yongshan Zhou, Monier Alhadi Abdelrahman Mohammed |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Biomedical Engineering Nanoparticle Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Catalysis 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Drug Discovery 030304 developmental biology 0303 health sciences Aqueous solution biology Process Chemistry and Technology General Medicine Enzyme assay Penicillin G Acylase chemistry Biocatalysis Transmission electron microscopy biology.protein Molecular Medicine Glutaraldehyde Biotechnology Nuclear chemistry |
| Zdroj: | Biotechnology and Applied Biochemistry. 69:629-641 |
| ISSN: | 1470-8744 0885-4513 |
| DOI: | 10.1002/bab.2138 |
| Popis: | In this work, Fe3 O4 nanoparticles (NPs) were coated with polydopamine (PDA) to structure Fe3 O4 @PDA NPs by the spontaneous oxygen-mediated self-polymerization of dopamine (DA) in an aqueous solution of pH = 8.5. The fabricated Fe3 O4 @PDA NPs were grafted by glutaraldehyde to realize the immobilization of penicillin G acylase (PGA) under mild conditions. The carriers of each stage were characterized and investigated by transmission electron microscopy, X-ray diffraction, Fourier transform infrared, and vibrating sample magnetometry. To improve the catalytic activity and stability of immobilized PGA, the immobilization conditions were investigated and optimized. Under the optimal immobilization conditions, the enzyme loading capacity, enzyme activity, and enzyme activity recovery of immobilized PGA were 114 mg/g, 26,308 U/g, and 78.5%, respectively. In addition, the immobilized PGA presented better temperature and pH stability compared with free PGA. The reusability study ensured that the immobilized PGA showed an excellent repeating application performance. In particular, the recovery rate of immobilized PGA could reach 94.8% and immobilized PGA could retain 73.0% of its original activity after 12 cycles, indicating that the immobilized PGA exhibited a high operation stability and broad application potential in the biocatalysis field. |
| Databáze: | OpenAIRE |
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