Transformation studies ofActinidia chinensisPlanch

Autor:	Catherine F. Harvey, Jacqueline Kent, Lena G. Fraser
Rok vydání:	1995
Předmět:	Actinidia deliciosa Actinidia chinensis biology Gene transfer Agrobacterium tumefaciens Horticulture biology.organism_classification law.invention Transformation (genetics) law Botany Shoot Agronomy and Crop Science Gene Polymerase chain reaction
Zdroj:	New Zealand Journal of Crop and Horticultural Science. 23:407-413
ISSN:	1175-8783 0114-0671
DOI:	10.1080/01140671.1995.9513917
Popis:	Transformed plants of four different genotypes of Actinidia chinensis Planch, were recovered from experiments using the Agrobacterium tumefaciens strains A281 and C58, both carrying the binary vector pKIWI105. A. chinensis was compared to a related species A. deliciosa, in its amenability to transformation and regeneration procedures. During regeneration A. chinensis shoot initiation was more rapid and prolific than A. deliciosa, although A. chinensis was more sensitive to small changes in growth regulator concentration. Comparisons between the different genotypes of A. chinensis showed only minor differences in growth regulator preferences. The strains of Agrobacterium tumefaciens that were effective as gene transfer agents in A. chinensis were the same as those which transformed A. deliciosa. Southern analysis and Polymerase Chain Reaction amplification of the transferred genes were used to confirm the transgenic nature of regenerated plantlets.
Databáze:	OpenAIRE
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