Development of Vacuoles and Vacuolar H+-ATPase Activity under Extremely High CO2Conditions in Chiorococcum littorale Cells

Autor:	M. Maeshima, N. A. Pronina, Takayuki Sasaki, Shigetoh Miyachi, I. Iwasaki, Norihide Kurano
Rok vydání:	1999
Předmět:	biology ATPase Protein subunit Plant Science General Medicine Vacuole Acclimatization Cell biology Chlorococcum littorale Vacuolar H+-ATPase biology.protein Fluorescence microscope Atpase activity Ecology Evolution Behavior and Systematics
Zdroj:	Plant Biology. 1:68-75
ISSN:	1438-8677 1435-8603
DOI:	10.1111/j.1438-8677.1999.tb00710.x
Popis:	The number and cross-sectional area of vacuoles in Chlorococcum littorale cells visualized with a differential interfer ence fluorescence microscope increased after their transfer from air to 40% CO2. An immunological observation indicated that the level of subunit B of vacuolar H*ATPase also increased under 40% CO2 conditions. The activity of nitrate-sensitive ATP-ase associated with the vacuolar membrane was 2–fold higher in 40% CO2--grown cells than in air-grown cells. The effects of inhi bitors on the ATPase activity confirmed that these activities were derived from vacuolar-type H-ATPase. These results sug gest that vacuole development associated with that of vacuolar H+-ATPase occurred during the acclimatization of C. littorale cells to extremely high CO2 conditions.
Databáze:	OpenAIRE
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