In vitro and in silico inhibition of α-amylase, α-glucosidase, and aldose reductase by the leaf and callus extracts of Vernonia anthelmintica (L.) Willd

Autor:	Maya Rajan, Y. Anie, Linu Mathew, Vinaya Chandran, S. Shahena
Rok vydání:	2021
Předmět:	chemistry.chemical_classification Aldose reductase Chloroform biology fungi Ethyl acetate food and beverages High-performance liquid chromatography chemistry.chemical_compound Enzyme Complementary and alternative medicine chemistry Biochemistry Rhamnetin Callus biology.protein Amylase
Zdroj:	Advances in Traditional Medicine. 22:125-139
ISSN:	2662-4060 2662-4052
Popis:	In the present study, the leaf and callus extracts of Vernonia anthelmintica prepared in three different solvents (methanol, ethyl acetate, and chloroform) were assessed for inhibition of α-amylase (EC 3.2.1.1), α-glucosidase (EC 3.2.1.20) and aldose reductase (EC 1.1.1.21). Both the leaf and callus extracts showed remarkable inhibition against these enzymes. Alpha-amylase inhibition was the highest in ethyl acetate extract of calli and methanolic extract of leaves. Ethyl acetate extract of both leaf and calli showed significant alpha-glucosidase and aldose reductase inhibition. HR-LCMS analysis of ethyl acetate fraction of leaf extract showed the presence of the flavonol rhamnetin. Also, the HPLC chromatogram revealed the presence of rhamnetin in callus extract. Further, in silico docking studies of rhamnetin was done against the above-mentioned enzymes. The in silico analysis revealed that compound rhamnetin showed good enzyme inhibition.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::20429c69b475823abde818807fc8ba42 https://doi.org/10.1007/s13596-020-00533-8 Zobrazit plný text záznamu Full text from SpringerLink