Systems Biology Approaches Reveal a Multi-stress Responsive WRKY Transcription Factor and Stress Associated Gene Co-expression Networks in Chickpea
| Autor: | Sushil K. Chaturvedi, Khela Ram Soren, Narendra Singh, Pallavi Singh, Aravind Kumar Konda, Parasappa R. Sabale, Santosh Rathod, Shanmugavadivel P. Subramaniam |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine WRKY transcription factor Systems biology Biology 01 natural sciences Biochemistry Cell biology Stress (mechanics) 03 medical and health sciences Computational Mathematics 030104 developmental biology Expression (architecture) Genetics Molecular Biology Gene 010606 plant biology & botany |
| Zdroj: | Current Bioinformatics. 14:591-601 |
| ISSN: | 1574-8936 |
| DOI: | 10.2174/1574893614666190204152500 |
| Popis: | Background: Chickpea is a nutritional rich premier pulse crop but its production encounters setbacks due to various stresses and understanding of molecular mechanisms can be ascribed foremost importance. Objective: The investigation was carried out to identify the differentially expressed WRKY TFs in chickpea in response to herbicide stress and decipher their interacting partners. Methods: For this purpose, transcriptome wide identification of WRKY TFs in chickpea was done. Behavior of the differentially expressed TFs was compared between other stress conditions. Orthology based cofunctional gene networks were derived from Arabidopsis. Gene ontology and functional enrichment analysis was performed using Blast2GO and STRING software. Gene Coexpression Network (GCN) was constructed in chickpea using publicly available transcriptome data. Expression pattern of the identified gene network was studied in chickpea-Fusarium interactions. Results: A unique WRKY TF (Ca_08086) was found to be significantly (q value = 0.02) upregulated not only under herbicide stress but also in other stresses. Co-functional network of 14 genes, namely Ca_08086, Ca_19657, Ca_01317, Ca_20172, Ca_12226, Ca_15326, Ca_04218, Ca_07256, Ca_14620, Ca_12474, Ca_11595, Ca_15291, Ca_11762 and Ca_03543 were identified. GCN revealed 95 hub genes based on the significant probability scores. Functional annotation indicated role in callose deposition and response to chitin. Interestingly, contrasting expression pattern of the 14 network genes was observed in wilt resistant and susceptible chickpea genotypes, infected with Fusarium. Conclusion: This is the first report of identification of a multi-stress responsive WRKY TF and its associated GCN in chickpea. |
| Databáze: | OpenAIRE |
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