Butyrylcholinesterase and acetylcholinesterase activity and quantal transmitter release at normal and acetylcholinesterase knockout mouse neuromuscular junctions
| Autor: | Eric Krejci, Arnaud Chatonnet, Jasmina Minic, Jordi Molgó |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Pharmacology
0303 health sciences medicine.medical_specialty biology Acetylcholinesterase Neuromuscular junction Neostigmine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology medicine.anatomical_structure chemistry Postsynaptic potential Internal medicine Muscarinic acetylcholine receptor biology.protein medicine 030217 neurology & neurosurgery Butyrylcholinesterase Acetylcholine 030304 developmental biology Cholinesterase medicine.drug |
| Zdroj: | British Journal of Pharmacology. 138:177-187 |
| ISSN: | 0007-1188 |
| DOI: | 10.1038/sj.bjp.0705010 |
| Popis: | 1 The present study was performed to evaluate the presence and the physiological consequences of butyrylcholinesterase (BChE) inhibition on isolated phrenic-hemidiaphragm preparations from normal mice expressing acetylcholinesterase (AChE) and BChE, and from AChE-knockout mice (AChE(-/-)) expressing only BChE. 2 Histochemical and enzymatic assays revealed abundance of AChE and BChE in normal mature neuromuscular junctions (NMJs). 3 In normal NMJs, in which release was reduced by low Ca(2+)/high Mg(2+) medium BChE inhibition with tetraisopropylpyrophosphoramide (iso-OMPA) or bambuterol decreased ( approximately 50%) evoked quantal release, while inhibition of AChE with fasciculin-1, galanthamine (10, 20 micro M) or neostigmine (0.1-1 micro M) increased (50-80%) evoked quantal release. Inhibition of both AChE and BChE with galanthamine (80 micro M), neostigmine (3-10 micro M), O-ethylS-2-(diisopropylamino)ethyl-methylphosphono-thioate (MTP) or phospholine decreased evoked transmitter release (20-50%). 4 In AChE(-/-) NMJs, iso-OMPA pre-treatment decreased evoked release. 5 Muscarinic toxin-3 decreased evoked release in both AChE(-/-) and normal NMJs treated with low concentrations of neostigmine, galanthamine or fasciculin-1, but had no effect in normal NMJs pretreated with iso-OMPA, bambuterol, MTP and phospholine. 6 In normal and AChE(-/-) NMJs pretreatment with iso-OMPA failed to affect the time course of miniature endplate potentials and full-sized endplate potentials. 7 Overall, our results suggest that inhibition or absence of AChE increases evoked quantal release by involving muscarinic receptors (mAChRs), while BChE inhibition decreases release through direct or indirect mechanisms not involving mAChRs. BChE apparently is not implicated in limiting the duration of acetylcholine action on postsynaptic receptors, but is involved in a presynaptic modulatory step of the release process. |
| Databáze: | OpenAIRE |
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