Preparation of a Molecular Library of Branched β-Glucan Oligosaccharides Derived from Laminarin
| Autor: | Shunji Natsuka, Aki Tachibana, Noriko Suzuki, Wataru Sumiyoshi, Shin-ichi Nakakita |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Fluorophore biology Stereochemistry Oligosaccharide Branching (polymer chemistry) Laminaria digitata biology.organism_classification Fluorescence High-performance liquid chromatography 03 medical and health sciences chemistry.chemical_compound Laminarin 030104 developmental biology chemistry Glucan |
| Zdroj: | Journal of Applied Glycoscience. 65:45-49 |
| ISSN: | 1880-7291 1344-7882 |
| Popis: | To study the structure of β-glucans, we developed a separation method and molecular library of β-glucan oligosaccharides. The oligosaccharides were prepared by partial acid hydrolysis from laminarin, which is a β-glucan of Laminaria digitata. They were labeled with the 2-aminopyridine fluorophore and separated to homogeneity by size-fractionation and reversed phase high-performance liquid chromatography (HPLC). Branching structures of all isomeric oligosaccharides from trimers to pentamers were determined, and a two-dimensional (2D)-HPLC map of the β-glucan oligosaccharides was made based on the data. Next, structural analysis of the longer β-glucan oligosaccharide was performed using the 2D-HPLC map. A branched decamer oligosaccharide was isolated from the β-glucan and cleaved to smaller oligosaccharides by partial acid hydrolysis. The structure of the longer oligosaccharide was successfully elucidated from the fragment structures determined by the 2D-HPLC map. The molecular library and the 2D-HPLC map described in this study will be useful for the structural analysis of β-glucans. |
| Databáze: | OpenAIRE |
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