| Autor: |
Helen L. Collins, Gregory J. Bancroft, Lynette B. Sigola, Caroline E. Cross |
| Rok vydání: |
1995 |
| Předmět: |
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| DOI: |
10.1016/s0091-679x(08)61849-x |
| Popis: |
Publisher Summary This chapter provides an introduction to obtaining and manipulating macrophages in vitro and in vivo . The chapter discusses for harvesting primary macrophage populations from the common tissue sites, their cultivation in vitro , and performing assays of macrophage activity—namely, phagocytosis and expression of class II major histocompatibility complex (MHC) antigens. The use of macrophage cell lines offers the advantage of supplying large numbers of cells with a uniform phenotype under controlled conditions. These have been used successfully to study aspects of macrophage-pathogen interactions and can respond to exogenous cytokines such as interferon γ (IFNγ) in some circumstances. It is essential that levels of lipopolysaccharide (LPS) be kept to a minimum in any procedure involving the manipulation of macrophages in vitro because significant changes in cell phenotype and function can occur in the presence of picogram quantities of this material. Although LPS is a potent second signal for macrophage activation if added after priming of the cells with cytokines such as IFNγ, addition before the cytokine can actually inhibit some activation events in vitro . |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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