Selen und seine Verbindungen – Bestimmung von Selenozucker 1, Selenozucker 2, Selenomethionin, Selenoethionin, methylseleniger Säure, Se-Methylselenocystein und Se-Methylselenoglutathion in Urin mittels Umkehrphasen-Chromatographie-ICP-MS

Autor: Hildebrand, Jörg, Jäger, Thomas, Göen, Thomas, Michalke, Bernhard, Hartwig, Andrea, MAK Commission
Jazyk: němčina
Rok vydání: 2022
Předmět:
human biomonitoring
high-performance liquid chromatography with inductively coupled plasma-mass spectrometry
environmental exposure
Arbeitsplatz
Umweltbelastung
Analyses in Biological Materials
general population
HBM
Urin
Hintergrundbelastung
intoxication
biomonitoring method
Selen
ICP-MS
HPLC-ICP-MS
background exposure
selenium
biologisches Monitoring
Biomonitoringmethode
employee
Methode
speciation analysis
Hochleistungs-Flüssigkeitschromatographie mit induktiv gekoppelter Plasma-Massenspektrometrie
analytical method
Arbeiter
Vergiftung
Analysen in biologischem Material
urine
Humanbiomonitoring
Arbeitsplatzbelastung
poisoning
workplace
biological monitoring
Arbeitnehmer
Analysenmethode
Intoxikation
selenium species
biomonitoring
method
Speziesanalytik
HPLC
Selenspezies
workplace exposure
worker
Allgemeinbevölkerung
DOI: 10.34865/bi778249bd7_2or
Popis: The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed and verified the presented biomonitoring method. This analytical method is used to determine relevant weakly polar or ionic organic selenium species in urine. They are separated by reversed-phase chromatography and subsequently detected using inductively coupled plasma-mass spectrometry (ICP‑MS). Calibration is performed using standard solutions prepared in pooled urine, which are processed and analysed analogously to the samples. The analytical method allows for the selective and sensitive determination of seven selenium species without interferences by other species. The limits of quantitation for the determination of the individual species lie between 0.18 μg/l and 0.34 μg/l urine (as selenium) and are thereby sufficient to measure exposure levels which exceed the background exposure of the general population.
Databáze: OpenAIRE
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