Determination of carbohydrate-deficient transferrin in human serum with capillary zone electrophoresis
| Autor: | Ulrich Marti, Jean-Bernard Falmagne, François de l’Escaille, Christian Lanz, Wolfgang Thormann |
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| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Organic Chemistry Carbohydrate deficient transferrin General Medicine Biochemistry Analytical Chemistry Blood serum Capillary electrophoresis Affinity chromatography chemistry Transferrin Spin column-based nucleic acid purification biology.protein Antibody Protein A |
| Zdroj: | Journal of Chromatography A. 1206:33-40 |
| ISSN: | 0021-9673 |
| Popis: | Capillary zone electrophoresis (CZE) in fused-silica capillaries is an effective analytical approach for the separation and determination of the transferrin (Tf) isoforms and thus carbohydrate-deficient transferrin (CDT) in human serum. Sera of patients with progressed liver cirrhosis are prone to interferences in the beta region which prevent the proper determination of CDT by CZE without additional sample preparation. Efforts to identify, reduce or even eliminate these interferences have been undertaken. Data obtained by ultrafiltration, affinity subtraction procedures using protein A, protein L and antibodies against immunoglobulins or Tf, and immunopurification of Tf suggest that the interferences in the patient sera are caused by increased levels of IgA and IgM and are best eliminated by immunopurification. Avian IgY antibody spin column immunocapture of serum Tf followed by CZE analysis of the stripped and concentrated fraction is shown to provide an attractive approach for CDT monitoring in sera with beta region interferences. |
| Databáze: | OpenAIRE |
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