Single and combined effects of colchicine, L-proline and post-inoculation low temperature on anther culture of wheat, Triticum aestivum L

Autor: B. Büter, J. E. Schmid, T. Attia, Peter Stamp, Amina Redha
Rok vydání: 1998
Předmět:
Zdroj: Plant Breeding. 117:335-340
ISSN: 1439-0523
0179-9541
DOI: 10.1111/j.1439-0523.1998.tb01950.x
Popis: This investigation was undertaken to study the effects of colchicine, L-proline and a post-inoculation cold temperature of 14°C for 7 days, either as a single or as combined treatments, on the parameters of anther culture of the DH83Z118.32 wheat genotype. Results indicate that the addition of 100 mg/1 colchicine to the induction medium for a period of 3 days and at an incubation temperature of 28°C yielded the highest success index. This treatment, although it significantly reduced embryogenesis, improved embryoid quality so that not only the total regeneration, but also the percentage of green plantlets increased significantly, In addition, it raised the percentage of doubled haploids and, consequently, led to a significantly better final success index. Combining the colchicine treatment with a reduced post-inoculation temperature did not intensify its beneficial effect, although a treatment of reduced temperature alone was superior to normal temperature (28°C) for most parameters studied. The beneficial effects of adding L-proline (200 mg/1) to the induction medium, combined with a low temperature (10°C for 4 days) treatment, were diminished when this treatment was combined with a colchicine treatment.
Databáze: OpenAIRE
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