Gene Expression Profiles of Odorant Binding Proteins inGlossina brevipalpis(Diptera: Glossinidae)

Autor: Rosaline W. Macharia, Esther Magiri, Daniel K. Masiga, Mary Murithi, Steven G. Nyanjom
Rok vydání: 2018
Předmět:
Zdroj: Journal of Entomological Science. 53:467-477
ISSN: 0749-8004
Popis: Glossina spp. (Diptera: Glossinidae) vector trypanosomes, the causal pathogens of trypanosomiasis in humans and animals. Glossina spp. chemosensory mechanism is relevant to understanding the behavioral responses of these insects to environmental cues. Odorant binding proteins (OBPs) are involved in either olfactory or nonolfactory cue sensation depending on their spatial and temporal expression. In most insects, the OBPs are ubiquitously expressed, although the levels are higher in the antennae. This study was aimed at determining the differential gene expression patterns of OBP genes in the developmental stages (larval, pupal, teneral) and differentially starved adults of Glossina brevipalpis (Newstead) collected from Shimba Hills National Park, Kenya. Five OBP genes, GbrOBP2, GbrOBP6, GbrOBP7, GbrOBP8, and GbrOBP13, were selected for quantitative PCR expression analysis as representatives of the three OBP classes in Glossina spp. (classic, minus-C, classic-dimers). Antennal samples from the ne...
Databáze: OpenAIRE