| Popis: |
Phenolic compounds are listed under priority pollutants by US EPA, and their concentration in effluent of coke oven industries reached to 1000 mg l-1 . However, investigation on biodegradation of phenolics from coke oven wastewater is found to be scanty. Therefore, an indigenous pseudomonas species with high toxicity tolerance and phenolic degradation abilities has been isolated from coke oven wastewater. In order to enhance the efficiency of phenol and to the study the interaction effect exists among them the significant media components are optimized employing response surface methodology (RSM) and central composite design (CCD RSM. Result reveals that MgSO4 plays considerable influence on biodegradation of phenol degradation by the indigenous pseudomonas species. Further, among the three independent variables, interaction effect between MgSO4 and FeCl3 found to be significant on degradation of phenol. The optimum values of the media constituents are as follows: 0.094 mgl-1 of NaCl, 0.019 mgl-1 FeCl3 and 0.1681 mgl-1 of MgSO4. Almost completely biodegradation of phenol is achieved at 900 mgl-1 of phenol within a short time period of 24h. Further, at this optimized culture condition almost completely degradation of phenol was achieved even at a very high initial concentration of 1500 mgl-1 within a shorter time period of 95h. Thus, indigenous Pseudomonas species found to be a potential candidate for degradation of high concentration of phenol from of coke oven wastewater |
