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Mononuclear cells were isolated from the central nervous system (CNS), lymph nodes (LN), spleen and blood, over the course of murine monophasic experimental autoimmune encephalomyelitis (EAE). Individual cytokine secreting T cells were enumerated. IL-2-secreting αβ T cells were numerous at all sites at disease onset. By disease peak their numbers had fallen profoundly; they remained low thereafter. IL-2 secreting γδ T cells were rare throughout. IFN-γ-secreting cells were plentiful at all sites at disease onset. γδ T cells comprised 7% of total and 20% of IFN-γ-secreting CNS-derived cells at disease onset; values at disease peak were 12 and 40% respectively. IL-4-secreting αβ T cells were rare in the CNS and LN throughout and did not increase in the spleen from baseline values. In contrast, splenic IL-4-secreting γδ T cells had increased to four-fold baseline values at disease onset and seven-fold at disease peak. Recovery from EAE is associated with a global inhibition of IL-2-secreting αβ T cells and to a lesser extent with IFN-γ-secreting αβ and γδ T cells, whereas IL-4-secreting γδ T cells increase in the spleen as disease evolves. |
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