RNA synthesis in isolated tobacco callus nuclei, and the influence of phytohormones
| Autor: | K. R. Libbenga, M.P.M. Van der Burg, A.M. Mennes, H. Bouman |
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| Rok vydání: | 1978 |
| Předmět: |
inorganic chemicals
chemistry.chemical_classification food and beverages Soil Science RNA RNA polymerase I activity Biology Molecular biology chemistry.chemical_compound chemistry Biochemistry Auxin RNA polymerase Callus Cytokinin Nucleoside triphosphate biology.protein Agronomy and Crop Science Polymerase |
| Zdroj: | Plant Science Letters. 13:329-339 |
| ISSN: | 0304-4211 |
| DOI: | 10.1016/0304-4211(78)90210-9 |
| Popis: | By an improved method nuclei were isolated from tobacco pith callus tissues and their RNA polymerase (nucleoside triphosphate: RNA nucleotidyltransferase (DNA-dependent), EC 2.7.7.6) activity was studied. The α-amanitin-sensitive polymerase activity in the nuclei was highest at high ionic strength (175 mM(NH4)2SO4) and was stimulated more by Mg2+ than by Mn2+. In the presence of 1 μg/ml of α-amanitin the assumed RNA polymerase I activity in the nuclei was maximal without (NH4)2SO4 and showed a preference for Mn2+ over Mg2+. Using [γ-32P] GTP as a label we proved that the isolated nuclei were able to initiate the synthesis of new RNA chains. Phytohormones (auxin and/or cytokinin) did not increase the incorporation of radioactive UMP in the nuclei, whether these were isolated in the presence or absence of the hormones. |
| Databáze: | OpenAIRE |
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