Clonogenicity of Human Endometrial Epithelial and Stromal Cells1

Autor:	Kjiana Elkje Schwab, Rachel Wah Shan Chan, Caroline E. Gargett
Rok vydání:	2004
Předmět:	Stromal cell Regeneration (biology) Cell Biology General Medicine Biology Endometrium Epithelium medicine.anatomical_structure Reproductive Medicine Stroma Immunology medicine Cancer research Stem cell Clonogenic assay Adult stem cell
Zdroj:	Biology of Reproduction. 70:1738-1750
ISSN:	1529-7268 0006-3363
DOI:	10.1095/biolreprod.103.024109
Popis:	The human endometrium regenerates from the lower basalis layer, a germinal compartment that persists after menstruation to give rise to the new upper functionalis layer. Because adult stem cells are present in tissues that undergo regeneration, we hypothesized that human endometrium contains small populations of epithelial and stromal stem cells responsible for cyclical regeneration of endometrial glands and stroma and that these cells would exhibit clonogenicity, a stem-cell property. The aims of this study were to determine 1) the clonogenic activity of human endometrial epithelial and stromal cells, 2) which growth factors support this clonogenic activity, and 3) determine the cellular phenotypes of the clones. Endometrial tissue was obtained from women undergoing hysterectomy. Purified single- cell suspensions of epithelial and stromal cells were cultured at cloning density (300–500/cm2) in serum medium or in serum- free medium supplemented with one of eight growth factors. Small numbers of e...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1f8d6a5e2bb4306bb01f370d43c80c09 https://doi.org/10.1095/biolreprod.103.024109 Zobrazit plný text záznamu