Purification of Soybean Mosaic Virus by Affinity Chromatography Using Monoclonal Antibodies

Autor:	John H. Hill, D. P. Durand, R. Diaco
Rok vydání:	1986
Předmět:	Gel electrophoresis Antigenicity biology medicine.drug_class Soybean mosaic virus biology.organism_classification Monoclonal antibody Virology Virus chemistry.chemical_compound chemistry Affinity chromatography Plant virus medicine Agarose
Zdroj:	Journal of General Virology. 67:345-351
ISSN:	1465-2099 0022-1317
DOI:	10.1099/0022-1317-67-2-345
Popis:	Summary Immunoaffinity columns were prepared by covalently binding monoclonal antibodies specific for soybean mosaic virus (SMV) to an agarose support matrix. SMV, purified by binding and elution, contained no contaminating proteins detectable by SDS-polyacrylamide gel electrophoresis. Affinity-purified SMV was essentially identical to virus purified by standard procedures in its infectivity, u.v. absorbance spectrum ratio of A 280/A 260, sedimentation coefficient, electrophoretic pattern of coat protein, morphology and antigenicity. This method of purification is rapid, reproducible, and yields highly purified virus preparations. It may therefore be applicable in the purification of a wide variety of plant viruses.
Databáze:	OpenAIRE
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