Solubilized candida cell Wall β-glucan, CSBG, is an epitope of natural human antibody

Autor: Yoshiyuki Adachi, Noriko N. Miura, Nakaya Saito, Shinya Masuzawa, Ken-ichi Ishibashi, Tatsuya Suzuki, Noriko Yoshikawa, Masakazu Akashi, Naohito Ohno, Sachiko Nameda, Masaharu Yoshida
Rok vydání: 2003
Předmět:
Zdroj: Drug Development Research. 58:179-189
ISSN: 1098-2299
0272-4391
DOI: 10.1002/ddr.10158
Popis: We have recently developed a protocol to obtain a soluble Candida spp. β1, 3-glucan (CSBG) by sodium hypochlorite (NaClO) oxidation and subsequent dimethyl sulfoxide (DMSO) extraction. CSBG is composed mainly of β-1,3 and β-1,6-glucosidic linkages with a small amount of branching. Antibody to β-glucan is generally difficult to produce. In the present study, the specificity of the human sera to CSBG was examined by ELISA. Using CSBG-coated plates, sera from normal human volunteers showed high reactivity that was neutralized by adding soluble CSBG as a competitor. However, the reactivity could not be neutralized by a β-1,6 branched β-1,3-glucan, grifolan. Similar specificity of the immunoglobulin (Ig) was detected in commercially available γ-globulin prepared from pooled human sera. Comparing various standard glucans, a major epitope was present on β-1,6 glucan segment. Drug Dev. Res. 58:179–189, 2003. © 2003 Wiley-Liss, Inc.
Databáze: OpenAIRE
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