Standardization of House Dust Mite Allergen (A13D. Pteronyssinus)
| Autor: | K. Veleva, G. Nikolov, T. Antonova, B. Petrunov, V. Radenkova, M. Hristova, A. Michova, B. Taskov |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Biotechnology & Biotechnological Equipment. 21:464-467 |
| ISSN: | 1314-3530 1310-2818 |
| DOI: | 10.1080/13102818.2007.10817495 |
| Popis: | The introduction of new in vitro methods, recommended by the European pharmacopoeia (1), enrich the characterization and evaluation of the allergens. The standardization of house dust mite allergen (A13 Dermatophagoides pteronyssinus) is based on different methods for determination of its structure and biological activity. Five batches of allergen were standardized by determination of protein nitrogen, by isoelectric focusing and by assessment of major allergen Der p1 by ELISA. The biological activity of the A13 was evaluated by skin prick test (SPT), IgE-binding potential after competitive inhibition and its activation capabilities for in vitro basophil degranulation. These batches, produced from 2002 - 2006, were with identical protein distribution according their isoelectrical points. Der p1 and protein nitrogen quantities varied between 37 – 87 ng/ml and 39 - 34 mg %. The studied lots showed similar results in the IgE-binding properties in comparison with the reference at 50 % inhibition level and in fl ow cytometry test for allergen-specifi c basophil degranulation. All the batches were with similar biological activity and induced skin-allergic reactions, comparable with the in-house reference preparation and histamine hydrochloride standard 1 mg/ml. The data from these new, in vitro methods improve the results from the accepted standardization procedure and will be performed as routine in the evaluation of biological activity of the house dust mite and others allergens. |
| Databáze: | OpenAIRE |
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