Restoration of morphogenic potential in Nicotiana by somatic hybridisation

Autor:	Gabriella Lázár, László Menczel, Anna H. Nagy, Pál Maliga, Ferenc Joó
Rok vydání:	1977
Předmět:	Kanamycin Resistance biology Somatic cell fungi food and beverages Kanamycin Protoplast biology.organism_classification Cell biology Complementation Callus Botany Genetics medicine Nicotiana sylvestris Molecular Biology medicine.drug Nicotiana
Zdroj:	Molecular and General Genetics MGG. 157:291-296
ISSN:	1432-1874 0026-8925
Popis:	KR103 is a kanamycin resistant cell line of Nicotiana sylvestris, deficient in inducible shoot redifferentiation. The existence of a genetically unaltered inducibility factor in KR103 was tested by fusing protoplasts of this line with those of Nicotiana knightiana, which can not be induced to form shoots in callus culture. Selection of somatic hybrids was based on screening for kanamycin resistance and green pigmentation, factors originally separated in the parental lines. The hybrid nature of two lines (H1 and H2) was shown by studies on esterase, alcohol dehydrogenase and glucose-6-phosphate dehydrogenase isoenzymes. In the somatic hybrids shoot formation on inductive medium was restored, indicating that the inducibility factor had not been altered in KR103. Genetic complementation and induced regulatory changes due to genome interaction are discussed as possible explanations for the restoration of morphogenic potential.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1f7604e9fb916261bd67cfe4a42a5e8a https://doi.org/10.1007/bf00268665 Zobrazit plný text záznamu Full text from SpringerLink