Application of modified pyrolytic graphite electrode as a sensor in the simultaneous assay of adenine and adenosine monophosphate
| Autor: | Anoop Raj Singh Rana, Himanshu Chasta, Sanghamitra Chatterjee, Rajendra N. Goyal |
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| Rok vydání: | 2011 |
| Předmět: |
Adenosine monophosphate
Detection limit Reproducibility Materials science Calibration curve Inorganic chemistry Metals and Alloys Analytical chemistry Square wave voltammetry Carbon nanotube Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention chemistry.chemical_compound chemistry law Electrode Materials Chemistry Pyrolytic carbon Electrical and Electronic Engineering Instrumentation |
| Zdroj: | Sensors and Actuators B: Chemical. 156:198-203 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2011.04.017 |
| Popis: | A simple and sensitive method based on square wave voltammetry (SWV) at single-walled carbon nanotube (SWNT) modified edge plane pyrolytic graphite electrode (EPPGE) is proposed for the simultaneous determination of adenine and adenosine-5′-monophosphate (5′-AMP). The modified electrode exhibits remarkable electrocatalytic properties towards adenine and 5′-AMP oxidation with a peak potential of ∼850 and 1165 mV respectively. Linear calibration curves are obtained over the concentration range of 5–100 nM for adenine and 10–100 nM for 5′-AMP with sensitivity of 677 and 476 nA nM−1 for adenine and 5′-AMP respectively. The limit of detection for adenine and 5′-AMP was found to be 37 × 10−10 M and 76 × 10−10 M, respectively. The effect of pH revealed that the oxidation of adenine and 5′-AMP at SWNT modified EPPGE involved equal number of electrons and protons. The modified electrode exhibited high stability and reproducibility. |
| Databáze: | OpenAIRE |
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