The fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT

Autor:	Marshall A. Lichtman, James K. Brennan, Camille N. Abboud, F. H. Kirkpatrick, John F. DiPersio
Rok vydání:	1980
Předmět:	Isoelectric focusing Monocyte Immunology Cell Cell Biology Hematology Biology Biochemistry Molecular biology Cytosol medicine.anatomical_structure Cytoplasm Cell culture medicine Cell fractionation Polyacrylamide gel electrophoresis
Zdroj:	Blood. 56:717-727
ISSN:	1528-0020 0006-4971
DOI:	10.1182/blood.v56.4.717.717
Popis:	GCT, a human monocyte-like cell line, has been shown to release biochemically distinct colony-stimulating activities (CSAs) for mouse and human marrows. These appear to be periodate-sensitive proteins with critical disulfide bonds. One, of molecular weight 145,000 daltons, stimulates macrophagic colony growth and is related to a 30,000-dalton molecule that also stimulates mouse growth. A 30,000-dalton CSA for human marrow can be separated from the 30,000-dalton mouse CSA by isoelectric focusing and gradient polyacrylamide gel electrophoresis. This distinction agrees with our previous finding of differential neutralization with anti-human urinary CSF antibody. The 30,000-dalton CSAs stimulate neutrophil, neutrophil-monocyte, and eosinophil colony growth in human marrow but only neutrophil and neutrophil-monocyte colonies in the mouse. Subcellular fractionation of GCT cells indicates that there are pools of preformed CSAs primarily associated with the cell cytosol that have similar apparent molecular weights to their secreted counterparts.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1f6ed71ecc8cf0c63d96aa5856b50371 https://doi.org/10.1182/blood.v56.4.717.717 Zobrazit plný text záznamu