Development and study the performance of PBA cladding modified fiber optic intrinsic biosensor for urea detection

Autor: V. G. Pahurkar, G. G. Muley, S. N. Botewad
Rok vydání: 2016
Předmět:
Zdroj: AIP Conference Proceedings.
ISSN: 0094-243X
DOI: 10.1063/1.4946538
Popis: The fabrication and study of a cladding modified fiber optic intrinsic urea biosensor based on evanescent wave absorbance has been presented. The sensor was prepared using cladding modification technique by removing a small portion of cladding of an optical fiber and modifying with an active cladding of porous polyaniline-boric acid (PBA) matrix to immobilize enzyme-urease through cross-linking via glutaraldehyde. The nature of as-synthesized and deposited PBA film on fiber optic sensing element was studied by ultraviolet-visible (UV-vis) spectroscopy and X-ray diffraction (XRD) analysis. The performance of the developed sensor was studied for different urea concentrations in solutions prepared in phosphate buffer.
Databáze: OpenAIRE