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Objectif : L’etude menee a ete de contribuer a l’evaluation de la toxicite aigue et subaigue de l’extrait au vin de palme des graines de Cucurma longa Linn. (cucurma) Methodologie et Resultats : L’extrait a subi un screening phytochimique et sa qualite microbiologique approuvee suivant la Pharmacopee Europeenne. L’essai de toxicite aigue a ete mene sur des souris femelles de Mus musculus a la dose 2000 mg/kg. L’essai de toxicite subaigue a ete realise sur une periode de 28 jours, avec 4 lots de 6 rats (3 mâles et 3 femelles albinos de la souche Wistar). Le lot I a recu 1 ml/100 g d’eau distillee et les lots II, III et IV l’extrait aux doses 200, 400 et 800 mg/kg respectivement. Le screening phytochimique a revele la presence d’alcaloides, de tanins, phenols et sucres reducteurs. Les tests de qualite microbiologique n’ont revele aucun germe. L’administration a dose unique de l’extrait n’a entraine aucun deces. La DL 50 de l’extrait est donc superieure a 2000 mg/kg. A doses repetees pendant 28 jours, l’extrait a contribue a une croissance ponderale non significative chez les rats a toutes les la doses chez les rats mâles et femelles. En outre, il a engendre egalement chez une augmentation de l’aspartate aminotransferase (ASAT) et de l’alanine aminotransferase (ALAT) aux trois doses de l’extrait chez les deux. Conclusion et Application : L’etude a permis de montrer que l’extrait au vin de palme des rhizomes de Curcuma longa Linn . a une DL 50 superieure a 2000 mg/kg et est favorable a la production d’un medicament traditionnel ameliore, apres les tests precliniques et cliniques. Mots cles : Curcuma longa, rhizomes, vin de palme, toxicite, souris, rats Study of acute and subacute toxicity of extract from the palm wine of rhizomes of Curcuma longa Linn. Objective : The study was to contribute to the evaluation of the acute and subacute toxicity of the palm wine extract of seeds of Carica papaya Linn . Methodology and Results : The extract underwent a phytochemical screening and its microbiological quality approved according to the European Pharmacopoeia . The acute toxicity test was conducted in female Mus musculus mice at a dose of 2000 mg/Kg. The subacute toxicity test was conducted over a 28-day period, with 4 batches of 6 rats (3 males and 3 albinos of the Wistar strain). Lot I received 1 mL/100 g of distilled water and lots II, III and IV extracted at doses of 200, 400 and 800 mg/Kg respectively. Phytochemical screening revealed the presence of alkaloids, tannins, phenols and reducing sugars. Microbiological quality tests revealed no contamination. The single-dose administration of the extract resulted in no deaths. The LD 50 of the extract was therefore greater than 2000 mg/Kg. At repeated doses for 28 days, the extract contributed to insignificant weight growth in rats at all dose levels in male and female rats. In addition, it also resulted in an increase of ASAT and ALAT at all three doses of the extract in both. Conclusion and Application : The study showed that the palm wine extract of Curcuma longa Linn rhizomes has an LD 50 greater than 2000 mg/Kg and is favorable to the production of an improved traditional medicine, after the preclinical and clinical tests. Keywords : Curcuma longa , rhizomes, palm wine, toxicity, mice, rats |
