Purification and physicochemical properties of starch phosphorylase from yam (Dioscorea cayenensis)
| Autor: | Jacques Diopoh, Ilphate Hamdan |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
Starch phosphorylase biology Starch Dioscoreaceae Plant Science General Medicine biology.organism_classification Sepharose chemistry.chemical_compound Glycogen phosphorylase Enzyme chemistry Biochemistry Sephadex Genetics Agronomy and Crop Science Polyacrylamide gel electrophoresis |
| Zdroj: | Plant Science. 76:1-7 |
| ISSN: | 0168-9452 |
| DOI: | 10.1016/0168-9452(91)90211-p |
| Popis: | Cytoplasmic starch phosphorylase from yam ( Dioscorea cayenensis ) was partially purified by ammonium sulfate fractionation. chromatography on DEAE A-50, Sephadex G-100 and Sepharose 6B filtration. Only one band showing phosphorylasic activity was found upon polyacrylamide gel electrophoresis. Its apparent molecular weight was 200 000 ± 3000. The enzyme is specific for starch and glucose 1-phosphate; it requires starch for its activity in the synthesis way. The enzymatic activity was inhibited by Hg 2+ and EDTA at high concentrations. Glucose did not inhibit the reaction. Thiol and Tyrosyl groups are required for enzymatic activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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