Aneuploidy Screening using Next Generation Sequencing
| Autor: | Milena Jakubowska, Tristan Darvin, Cengiz Cinnioglu, Refik Kayali, Adedoyin Akinwole, Gary Harton |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Assisted reproductive technology Spontaneous miscarriage medicine.diagnostic_test medicine.medical_treatment 010401 analytical chemistry Aneuploidy Embryo Blastomere Biology medicine.disease 01 natural sciences DNA sequencing 0104 chemical sciences Andrology 03 medical and health sciences 030104 developmental biology Implantation failure medicine Genetic testing |
| Zdroj: | Prenatal Diagnosis ISBN: 9781493988877 |
| DOI: | 10.1007/978-1-4939-8889-1_6 |
| Popis: | Chromosomal aneuploidy is recognized to be a significant contributing factor in implantation failure and spontaneous miscarriage Hellani et al. (Reprod Biomed Online 17:841-847, 2008), Vanneste et al. (Nat Med 15:577-583, 2009) and is likely to be responsible for the majority of IVF failure [Baltaci et al. (Reprod Biomed Online 12:77-82, 2006), Munne (Placenta 24:S70-76, 2003)]. Preimplantation genetic testing for aneuploidy (PGT-A) screening, formerly termed preimplantation genetic screening (PGS), enables the assessment of the numeric chromosomal constitution in blastomere and/or trophectoderm biopsy before embryo transfer.Preimplantation genetic testing for aneuploidy (PGT-A) has been proven to improve the selection of embryos for transfer and therefore also assisted reproductive technology (ART) cycles. In this chapter we describe the current gold standard platform for PGT-A, next generation sequencing (NGS) protocol used in our laboratory. |
| Databáze: | OpenAIRE |
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