Copper tolerance of the soft-rot fungus Phialophora malorum grown in-vitro revealed by microscopy and global protein expression
| Autor: | Geoffrey Daniel, Jonas Bergquist, Jean Pettersson, Jia Mi, Hasanthi Karunasekera |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification biology Hypha fungi 030106 microbiology chemistry.chemical_element Fungus 010501 environmental sciences biology.organism_classification Polysaccharide 01 natural sciences Microbiology Copper Staining Biomaterials Cell wall 03 medical and health sciences chemistry Biochemistry Extracellular Waste Management and Disposal Mycelium 0105 earth and related environmental sciences |
| Zdroj: | International Biodeterioration & Biodegradation. 137:147-152 |
| ISSN: | 0964-8305 |
| DOI: | 10.1016/j.ibiod.2018.12.001 |
| Popis: | In this study, we used proteomics in conjunction with microscopy to study differences in the proteome and hyphal morphology of the copper tolerant soft rot fungus Phialophora malorum grown in media containing 0.064, 0.64% Cu as CuSO4. Unique proteins were found in the control and the copper-treated (0.064% CuSO4) samples. Of five unique proteins found in the 0.064% CuSO4 treated cultures, ATP synthase subunit alpha is considered to play an important role in copper tolerance as it is involved in the biosynthesis of fatty acids and steroids and may relate to morphological changes associated with hyphal cell walls of the fungus when grown in the presence of copper. ICP-AES analyses showed total mycelial Cu to increase with media Cu with 5246- and 16535 μg Cu/g dry wt mycelia respectively found in 0.064 and 0.64% Cu-cultures after 6 weeks growth. Rubeanic acid staining of 0.064% mycelia showed Cu bound in intracellular bodies while most Cu was found as extracellular precipitates on the surfaces of hyphae in 0.64% Cu. SEM showed hyphal surfaces enrobed in fibrillar polysaccharides to which Cu was bound. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect