Continuous production of fructooligosaccharides and invert sugar by chitosan immobilized enzymes: Comparison between in fluidized and packed bed reactors

Autor:	Luiza F. Aydos, Plinho Francisco Hertz, André Soibelmann Glock Lorenzoni, Marco Antônio Záchia Ayub, Manuela P. Klein, Rafael C. Rodrigues
Rok vydání:	2015
Předmět:	Packed bed Sucrose Chromatography Immobilized enzyme Process Chemistry and Technology Bioengineering equipment and supplies Biochemistry Catalysis Continuous production chemistry.chemical_compound Hydrolysis chemistry Fluidized bed Glutaraldehyde Sugar
Zdroj:	Journal of Molecular Catalysis B: Enzymatic. 111:51-55
ISSN:	1381-1177
DOI:	10.1016/j.molcatb.2014.11.002
Popis:	In this work, β-fructofuranosidase and β-fructosyltransferase were covalently immobilized on chitosan spheres, using glutaraldehyde as a coupling agent, in order to produce invert sugar and fructooligosaccharides (FOS). Maxinvert L was used to make β-fructofuranosidase biocatalyst yielding 7000 HU/g. A partial purified β-fructosyltransferase from Viscozyme L was used to prepare the other biocatalyst yielding 2100 TU/g. The production of invert sugar and FOS was evaluated using different continuous enzymatic reactors: two packed bed reactors (PBR) and two fluidized bed reactors (FBR). The invert sugar production achieved a yield of 98% (grams of product per grams of initial sucrose) in the PBR and 94% in the FBR, whereas FOS production achieved a yield of 59% in the PBR and 54% in the FBR. It was also observed in both cases that varying the flow rate it is possible to modulate the FOS composition in terms of nystose and kestose concentrations. The operational stability of FOS produced in the PBR was evaluated for 40 days showing no reductions in yields.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1f47b42daaa2f46738787e51be66b267 https://doi.org/10.1016/j.molcatb.2014.11.002 Zobrazit plný text záznamu Full Text from ScienceDirect