The Phenotypic Conversion of Macrophage Detected by FACS in EAE Mice Treated with or without Fasudil

Autor:	Yan-Hua Li, Jie-Zhong Yu, Yong Xie, Baoguo Xiao, Shaowei Hou, Cun-Gen Ma, Chun-Yun Liu, Ling Feng, Hai-fei Zhang
Rok vydání:	2013
Předmět:	CD40 medicine.diagnostic_test biology Chemistry CD14 Fasudil CD11c Peripheral blood mononuclear cell Flow cytometry Immunology medicine TLR4 biology.protein Macrophage
Zdroj:	Engineering. :491-495
ISSN:	1947-394X 1947-3931
DOI:	10.4236/eng.2013.510b101
Popis:	We studied the changes of macrophage populations in splenic mononuclear cells of experimental autoimmune ence-phalomyelitis (EAE) mice treated with or without Fasudil. Phenotypic analysis using flow cytometry showed that the levels of TLR4, CD11c and CD40 which represent the type 1 macrophage, were depressed in Fasudil-treated mice. Incontrast, it was observed the expressions of CD200 and CD14 which typify the type 2 macrophage were elevated in Fasudil-treated mice as compared to EAE mice. And we also found that Fasudil at dose of 40 mg/kg alleviated the se-verity of symptom in EAE mice. Based on the evidence that M1 macrophages are neurotoxic and M2 macrophages promote a regenerative growth, indicating that polarization and shifting of macrophages into M2 cells may also play key roles in treatment of EAE.
Databáze:	OpenAIRE
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