Integration of HBV-DNA may not be a prerequisite for the maintenance of the state of malignant transformation
| Autor: | Chia-Ming Chu, J. Monjardino, C. Greenfield, M. J. F. Fowler, Eng-Kiong Yeoh, Peter Karayiannis, B. M. Wankya, Anna S.F. Lok, Ching-Lung Lai, A.A. Dunk, H.C. Thomas |
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| Rok vydání: | 1986 |
| Předmět: |
Hepatitis B virus
medicine.medical_specialty HBsAg Hepatology medicine.diagnostic_test business.industry Hepatobiliary disease virus diseases medicine.disease Chronic liver disease medicine.disease_cause Gastroenterology digestive system diseases Malignant transformation Liver disease Liver biopsy Internal medicine Immunology Biopsy medicine business |
| Zdroj: | Journal of Hepatology. 2:218-229 |
| ISSN: | 0168-8278 |
| DOI: | 10.1016/s0168-8278(86)80080-0 |
| Popis: | Summary Hundred and ten liver biopsy specimens from various parts of the world were examined for episomal and integrated HBV-DNA sequences. In 54 patients with HBsAg chronic liver disease episomal HBV-DNA was found in 83% of HBeAg-positive patients, compared to only 22% of patients with anti-HBe. Furthermore episomal HBV-DNA in the latter predominated among the Asians. Integrated HBV-DNA was found only in 5.5% of HBeAg-positive patients but in 16.5% of patients with anti-HBe. In 28 HBsAg-positive patients with hepatoma, episomal HBV-DNA was found in 50% of HBeAg-positive patients but in only 11% of anti-HBe patients. Conversely integrated sequences were less common (25%) in HBe-Ag-positive patients than in anti-HBe patients (50%), giving an overall incidence of integration in this group of 45%. No episomal, and only one case of integrated sequences of HBV-DNA, could be detected among 10 patients with HBsAg-negative hepatoma. In addition neither episomal nor integrated HBV-DNA could be detected in 18 patients with non-HBV-related liver disease. Our data suggests that stable integration of HBV-DNA into the host's genome is not necessarily a prerequisite for the maintenance of the state of malignant transformation but may be necessary for its initiation. Alternatively, the detection of integrated HBV-DNA may represent a ‘snap shot' of a random integration event amplified by clonal expansion promoted by other factors. |
| Databáze: | OpenAIRE |
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