[Letter to the editor] The selenium content of cell culture serum influences redox-regulated gene expression

Autor:	Kathryn F. Tonissen, Wei-Che Yu, Ujang Tinggi, Therese Karlenius, Hye-Jin Hawkes, Francis Michael Clarke, Fenil Shah
Rok vydání:	2011
Předmět:	Genetics chemistry.chemical_element Tumor cells Biology Molecular biology General Biochemistry Genetics and Molecular Biology Penicillin chemistry Breast cancer cell line Streptomycin Cell culture Gene expression medicine Selenium Serum chemistry Biotechnology medicine.drug
Zdroj:	BioTechniques. 50:295-301
ISSN:	1940-9818 0736-6205
DOI:	10.2144/000113666
Popis:	Cell Culture The MDA-MB-231 breast cancer cell line (see Reference 24 in the main text) was cultured in RPMI 1640 medium (Invitrogen, Melbourne, Australia), supplemented with either 10% FBS [either Invitrogen, Lonza (Melbourne, Australia), Quantum (Brisbane, Australia), or 10% serum supreme (Lonza) and 100 μg/mL penicillin (Invitrogen) and 100 μg/mL streptomycin (Invitrogen) in 5% CO2 at 37°C. Media used in transfections did not contain penicillin or streptomycin. All chemicals were purchased from SigmaAldrich (Castle Hill, Australia) unless otherwise specified.
Databáze:	OpenAIRE
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