Community Analysis of Seed-Associated Microbes in Forage Crops using Culture-Independent Methods
| Autor: | Hiroshi Ezura, Kiwamu Minamisawa, Toshiro Sato, Seishi Ikeda, Nozomi Ytow, Shin Ichi Fuji, Tatsuhito Fujimura |
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| Rok vydání: | 2006 |
| Předmět: |
biology
Ribosomal Intergenic Spacer analysis Dioszegia food and beverages Soil Science Pseudomonas fluorescens Plant Science General Medicine Lolium multiflorum biology.organism_classification Microbial population biology Botany Environmental DNA Clavibacter michiganensis Ecology Evolution Behavior and Systematics Cladosporium |
| Zdroj: | Microbes and Environments. 21:112-121 |
| ISSN: | 1347-4405 1342-6311 |
| Popis: | Microbial diversity in forage crop seeds was examined using culture-independent methods. Environmental DNA samples were extracted from the surface of the seeds, and the intergenic spacer region between small subunit and large subunit RNA genes was then amplified by ribosomal intergenic spacer analysis (RISA) for the profiling of microbial community structures. The results suggested the presence of stable microbial communities in seeds and sequencing of the RISA amplicons identified a total of 33 unique microbial sequences in 1.6 g of seed material from Italian ryegrass (Lolium multiflorum) and timothy (Phleum pretense). These included several sequences showing high similarity to known plant-associated microbes, such as Pseudomonas fluorescens and Clavibacter michiganensis, the Cladosporium and Dioszegia group of fungi, and also several uncultured bacteria. |
| Databáze: | OpenAIRE |
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