Presence of complementary RNA in chicken sarcoma cells and Rous sarcoma virus
| Autor: | É. A. Ratovitskii, O. K. Kuznetsov, Ya. D. Shaposhnikov |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
chemistry.chemical_classification
Rous sarcoma virus animal structures Ribonucleotide viruses RNA General Medicine Biology biology.organism_classification Subcellular localization medicine.disease Molecular biology General Biochemistry Genetics and Molecular Biology Virus chemistry Polysome embryonic structures medicine Nucleotide Sarcoma |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 85:356-358 |
| ISSN: | 1573-8221 0007-4888 |
| Popis: | The subcellular localization of nucleotide sequences in Rous chicken sarcoma complementary to RNA of Rous sarcoma virus was investigated by RNA-RNA molecular hybridization. Samples of virion RNA labeled with radioactive iodine (125I) were annealed with RNA from different fractions (nuclei, mitochondria, free and membrane-bound polysomes) isolated from Rous chicken sarcoma cells. The formation of RNase-resistant hybrids between virus RNA-125I and RNA from mitochondria and membrane-bound polysomes was demonstrated; the relative frequency of occurrence of sequences complementary to virion RNA in the latter, moreover, was 446 times greater than in the former. The role of complementary ribonucleotide sequences is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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