Enzyme amplification for immunoassays
| Autor: | Axel Johannsson, David L. Bates, David H. Ellis, Anne M. Plumb, Christopher John Stanley |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
chemistry.chemical_classification
Detection limit Chromatography medicine.diagnostic_test biology Immunology chemistry.chemical_compound Enzyme chemistry Biochemistry Diaphorase Immunoassay medicine biology.protein Immunology and Allergy Alkaline phosphatase NAD+ kinase Formazan Alcohol dehydrogenase |
| Zdroj: | Journal of Immunological Methods. 87:7-11 |
| ISSN: | 0022-1759 |
| Popis: | A method is described for increasing the response of enzyme immunoassays employing alkaline phosphatase as the label initiating 2 sequential catalytic reactions. First, NADP is dephosphorylated to produce NAD, which catalytically activates a specific redox-cycle involving the enzymes alcohol dehydrogenase and diaphorase. During each turn of the cycle 1 molecule of a tetrazolium salt is reduced to an intensely coloured formazan. The method is capable of detecting as little as 0.01 amol alkaline phosphatase, and when applied to an immunoassay for TSH a sensitivity (zero + 2.5 standard deviations) of 0.0013 mIU/l was obtained. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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