Phytochemical prospecting, isolation, and protective effect of the ethanolic extract of the leaves of Jatropha mollissima (Pohl) Baill
| Autor: | Kelvim Crist Araújo Rocha, Cleidiane Macêdo Santos, João Gabriel Silva Morais, Adriane da Cunha Aragão Rios Fagundes, José Rafael da Silva Araujo, Francielle Alline Martins, Pedro Marcos de Almeida, Francisco Artur e Silva Filho |
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| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Mitotic index biology Traditional medicine Chemistry Antiparasitic medicine.drug_class Health Toxicology and Mutagenesis Jatropha Fractionation Toxicology biology.organism_classification Antimicrobial 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Spinasterol Phytochemical 030220 oncology & carcinogenesis medicine Allium 030304 developmental biology |
| Zdroj: | Journal of Toxicology and Environmental Health, Part A. 84:743-760 |
| ISSN: | 1087-2620 1528-7394 |
| DOI: | 10.1080/15287394.2021.1938767 |
| Popis: | Jatropha mollissima is used in folk medicine as antimicrobial, antiparasitic, and larvicidal. However, few toxicogenetic studies have been carried out. Therefore, the aim of this study was to determine the phytochemical profile of ethanolic leaf extract of J. mollissima (EEJM) as well as potential cytotoxic, mutagenic, and antimutagenic properties. The EEJM was subjected to successive fractionation for the isolation of secondary metabolites, and five concentrations (0.01; 0.1; 1; 10 and 100 mg/ml) of extract were investigated using Allium cepa assay and the Somatic Mutation and Recombination (SMART) test. The mitotic index and % damage reduction were analyzed for A. cepa and the frequency of mutant hair for SMART. The presence of coumarins, alkaloids, flavonoids, saponins, and tannins was detected, while spinasterol and n-triacontane were the isolates identified for the first time for this species. EEJM did not exhibit cytotoxicity and was not mutagenic at 1 or 10 mg/ml using A. cepa and all concentrations of EEJM were not mutagenic in the SMART test. A cytoprotective effect was found at all concentrations. At 1 or 10 mg/ml EEJM exhibited antimutagenicity in A. cepa. In SMART, the protective effect was observed at 0.1 to 100 mg/ml EEJM. Our results demonstrate the important chemopreventive activity of EEJM, a desired quality in the search for natural anticarcinogenic compounds. |
| Databáze: | OpenAIRE |
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