| Popis: |
According to the World Health Organization (WHO) report, the world has experienced more than 568 million positive cases and more than 6 million deaths due to COVID infection. WHO declared a pandemic due to the rapid spread of the SARS-CoV-2 virus, and the fight against this pandemic is not over yet. The significant reasons for virus spread are the lack of detection kits, appropriate detection techniques, delay in detection, asymptomatic cases and failure in mass screening. In the last two years, several researchers and medical companies have introduced successful test kits to detect the infection of symptomatic patients in real-time, and it was needed to monitor the spreading. But it is equally important to have information on the asymptomatic cases, which can be done by antibody testing for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus. We have developed a simple, advantageous immobilization procedure for rapidly detecting SARS-CoV-2 spike protein in this work. Carbon-MEMS-derived glassy carbon (GC) is used as the sensor electrode, and the detection is based on covalently linking the SARS-CoV-2 antibody to the GC surface. Glutaraldehyde was a cross-linker between antibody and glassy carbon electrode (GCE). The binding was investigated using Fourier Transform Infrared Spectroscopy (FTIR) characterization and cyclic voltammetric (CV) analysis. Electrochemical impedance spectroscopy was utilized to measure the change in total impedance before and after incubation of the SARS-CoV-2 antibody with various concentrations of SARS-CoV-2 spike protein. It is observed that the developed sensor can sense 1 fg/ml to 1 µg/ml concentration of SARS-CoV-2 spike protein. This detection is label-free, and the chances of false alarm are almost nil. The developed sensor can be used for mass screening as it is cost-effective. |
