A novel composition for in vitro and in vivo regeneration of skin and connective tissues

Autor: Gian Franco Calisti, M. Panzone, Stefano Reguzzi, Elia Ricci, Giovanni Cavallo, Tetyana Denysenko, Maria Augusta Roos, Gianpiero Pescarmona, Manuela Martano, Rocco De Siena, Andrea Vercelli, Simona Fiobellot, Luisa Gabetti, Luisa Gennero
Rok vydání: 2011
Předmět:
Zdroj: Cell Biochemistry and Function. 29:311-333
ISSN: 0263-6484
DOI: 10.1002/cbf.1751
Popis: The particular combination of polydeoxyribonucleotides, l-carnitine, calcium ions, proteolytic enzyme and other ingredients acts in a synergetic way in the regeneration of skin and connective tissues. This new formulation of active principles was tested in vitro as a cell and tissue culture medium and in vivo for various preparations in support of tissue regeneration. In vitro, the new blend allowed the maintenance of skin biopsies for more than 1 year in eutrophic conditions. Immunocytochemical analyses of fibroblasts isolated from these biopsies confirmed a significant increase of the epidermal and connective wound-healing markers such as collagen type I, collagen type IV, cytokeratin 1 (CK1), CK5, CK10 and CK14 versus controls. To examine the effects of the new compound in vivo, we studied impaired wound healing in genetically diabetic db/db mice. At day 18, diabetic mice treated with the new composition showed 100% closure of wounds and faster healing than mice treated with the other solutions. This complex of vital continuity factors or life-keeping factors could be used as a tissue-preserving solution or a cosmetic/drug/medical device to accelerate wound healing in the treatment of patients with deficient wound repair to promote the regeneration of cutaneous and connective tissues (injuries-wound, dermatitis) and prevent the recurrent relapses.
Databáze: OpenAIRE
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