| Popis: |
The Polygonatum odoratum cv. Gungangbeaksea, bred in Gyeongsangnam-Do Agricutural Research & Extension Service, was cultured in vitro for micropropagate rapidly through the culture of rhizome explants (5 × 5 ㎜). The 7 × 7 ㎜ explants of adventitious multi-bud clusters (AMC), obtained through the culture of rhizome explants (MS + 3.0 ㎎/L BA) were cultured on MS media with BA and TDZ. The shoot multiplication was favorable on the MS medium containing 3.0 ㎎/L TDZ with 2.8 in shoot number. But the formation of AMC was low in all media tested. The explants of AMC were cultured on MS media containing 1.0~5.0 ㎎/L TDZ and NAA to multiplicate AMC more. The formation of AMC was a little more stimulated on combined MS media of TDZ and NAA, than that with TDZ alone. The multiplication of shoots and AMC was favorable on MS media with 3.0 ㎎/L TDZ and 5.0 ㎎/L NAA, and 5.0 ㎎/L TDZ and 3.0 ㎎/L NAA. As the concentration of MS salts increased, the formation of AMC was decreased. But the formation of AMC was more stimulated, as the concentration of sucrose increased to 7%. Therefore, the multiplication of shoots and AMC was suitable on media containing 3.0~5.0 ㎎/L TDZ and NAA, and 7% of sucrose. The explants of AMC were rooted on media with 3.0 ㎎/L IBA, or 2.0 ㎎/L NAA with more than 80% in rooting ratio. The plantlets were treated at 5 ℃ for 8 weeks, and cultured ex vitro for 8 weeks. The survival ratio of plantlets were 100% in vermiculite, and the mixed soil with perlite 1 volumn and vermiculite 1 volumn. |
