Deletion mutants of the B type natriuretic peptide receptor: cDNA expression and protein purification and characterization

Autor:	N. V. Gibanova, D. L. Kakuev, O. V. Solov’eva, Valery M. Lipkin
Rok vydání:	2000
Předmět:	Serine Biochemistry Kinase Organic Chemistry Protein purification Secretion Heterologous expression Biology NPR1 Receptor NPR2 Molecular biology
Zdroj:	Russian Journal of Bioorganic Chemistry. 26:390-397
ISSN:	1573-9163 1068-1620
DOI:	10.1007/bf02758666
Popis:	The C type natriuretic peptide (CNP) is a peptide hormone stimulating vasorelaxation and inhibiting cell proliferation. CNP activates the type B natriuretic peptide receptor (NPR-B), known as the guanylate cyclase B membrane enzyme, which results in the cGMP release. To study functional properties of NPR-B, its gene fragments were expressed in methylotrophic yeastsPichia pastoris. Conditions were found providing for secretion of functionally active recombinant proteins NPR-Bs and NPR-Bl into the cultural medium in a yield of 25 mg/l culture. Their specific activity was 0.97 and 0.93 µmol cGMP min−1 mg−1 protein, respectively. It was shown that NPR-B belongs to the family of Ser/Thr protein kinases and can be autophosphorylated at the serine residues.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1f12ea841cef7f43c34a8f38e0a9fb0c https://doi.org/10.1007/bf02758666 Zobrazit plný text záznamu Full text from SpringerLink