Effects of Colloidal Crystals, Antibiotics, and Surface-Bound Antimicrobials on Pseudomonas aeruginosa Surface Density
| Autor: | Joseph O. Falkinham, A. L. Ritter, Htwe H Mon, Yow-Ren Chang, William A. Ducker |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Colony-forming unit Chemistry Pseudomonas aeruginosa 030106 microbiology Biomedical Engineering Biofilm 02 engineering and technology Colloidal crystal 021001 nanoscience & nanotechnology Antimicrobial medicine.disease_cause Biomaterials 03 medical and health sciences Adsorption Monolayer Tobramycin medicine 0210 nano-technology medicine.drug Nuclear chemistry |
| Zdroj: | ACS Biomaterials Science & Engineering. 4:257-265 |
| ISSN: | 2373-9878 |
| DOI: | 10.1021/acsbiomaterials.7b00799 |
| Popis: | We examined the effect of a crystalline layer of silica particles in the size range 0.5–4 μm on the adsorption and surface growth of Pseudomonas aeruginosa. Growth on these colloidal crystal monolayers (CCMs) was compared to growth on a flat plate of silica. All surfaces were coated with a thin film of silica to provide chemical uniformity of the different topographies. The results showed that the CCM reduces the density of colony forming units (CFU) on the solid by 99–99.9% when the suspension load was 103 CFU. We also examined the interaction between the CCM and either antibiotics or a chemically bound antimicrobial. The addition of 20 μg/mL tobramycin after an initial 24 h growth period caused a further decrease in CFU counts of about 99–99.9% for all topographies. The percentage reduction as a result of the antibiotics was similar for all topographies, which suggested that there was no particular synergy between the topography and antibiotics. On the other hand, the additive nature of the two effects ... |
| Databáze: | OpenAIRE |
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