Isonitrile derivatives of polyacrylamide as supports for the immobilization of biomolecules
| Autor: | Adina Niv, Leon Goldstein |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Immobilized enzyme
Polyacrylamide Bioengineering General Medicine Applied Microbiology and Biotechnology Biochemistry Michaelis–Menten kinetics Solvent chemistry.chemical_compound chemistry Amide Proton transport Alkoxide Polymer chemistry Side chain Organic chemistry Molecular Biology Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 42:19-35 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/bf02788899 |
| Popis: | Isonitrile derivatives of crosslinked polyacrylamide beads (Biogell P-100) were prepared by a two-step procedure: a. N-hydroxymethylation (methylolation) of amide groups on the polymer by treatment with formaldehyde; and b. Attachment of side chains, containing isonitrile functional groups by a displacement reaction involving 1-tosyloxy-3-isocyanopropane (p-CH3-C6H4·SO2·O·(CH2)3 NC) and alkoxide ions generated on methylolated polyacrylamide by treatment with a strong base in a polar aprotic solvent. The modified polyacrylamide beads were tested as support for the immobilization of proteins, and low mol wt ligands by four component condensation (4CC) reactions. Trypsin-polyacrylamide acting on N-benzoyl-L-arginine ethylester exhibited nonlinear Michaelis Menten kinetics and distorted pH activity profiles. The kinetic anomalies could be reduced by increasing the concentration of buffer. The data were consistent with a model assuming “buffer facilitated proton transport” in a diffusionally constrained system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink