Purification of polyhydroxybutyrate produced by Burkholderia cepacia IPT64 through a chemical and enzymatic route
| Autor: | Alfredo Eduardo Maiorano, Daniel V. Suzuki, Maria Filomena de Andrade Rodrigues, Elda Sabino da Silva, José Marcio Carter |
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| Rok vydání: | 2007 |
| Předmět: |
inorganic chemicals
chemistry.chemical_classification Chromatography Lysis biology Physiology Sodium chemistry.chemical_element General Medicine biology.organism_classification Applied Microbiology and Biotechnology Polyhydroxybutyrate chemistry.chemical_compound Burkholderia Enzyme chemistry Reagent Organic chemistry Hydrogen peroxide Fenton's reagent Biotechnology |
| Zdroj: | World Journal of Microbiology and Biotechnology. 24:771-775 |
| ISSN: | 1573-0972 0959-3993 |
| DOI: | 10.1007/s11274-007-9537-x |
| Popis: | This work aimed at the study of purification of biopolymers produced by Burkholderia cepacia IPT64, through a chemical attack like an adjuvant procedure through chemical and enzymatic route. Enzymatic lysis using protease was run on an enzyme/cell ratio of 0.02. Chemical attacks were performed as pre or post-treatment for enzymatic attacks, using sodium dodecyl sulphate or hydrogen peroxide. The same chemicals and procedures were used alone as a control. Fenton’s reagent was also tested as a chemical treatment. Using only one of the chemicals H2O2, Fenton’s reagent or SDS, the purity increase achieved values of 14%, 16% or 23%, respectively. When H2O2 or SDS were used as pre-treatment for the enzymatic attack, the results of purity increase achieved values of 58% for H2O2/cell ratio between 0.60 and 1.20, and 57% when SDS/cell ratio at 0.56 was used. In the case when H2O2 or SDS were used as post-treatment for the enzymatic attack, results of purity increase achieved 60% when the H2O2 was used at H2O2/cell ratio ranging between 0.30 and 0.60 and 71% when SDS was used at a ratio SDS/cell of 0.56. |
| Databáze: | OpenAIRE |
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