Immobilization of Tyrosinase in Poly(2-thiophen-3-yl-alkyl ester) Derivatives
| Autor: | Senem Kiralp Kayahan, Levent Toppare, Pinar Camurlu |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
Conductive polymer Materials science Polymers and Plastics Immobilized enzyme Tyrosinase General Chemistry Polypyrrole Reaction rate chemistry.chemical_compound PEDOT:PSS chemistry Polymer chemistry Materials Chemistry Ceramics and Composites Biosensor Alkyl Nuclear chemistry |
| Zdroj: | Journal of Macromolecular Science, Part A. 45:1009-1014 |
| ISSN: | 1520-5738 1060-1325 |
| DOI: | 10.1080/10601320802454169 |
| Popis: | In this study, construction of novel biosensors for the determination of phenolic compound was performed via immobilization of tyrosinase during the electrochemical synthesis of conducting block copolymers of 2-thiophen-3-yl-alkyl ester derivatives with 3,4-ethylenedioxythiophene and synthesis of poly(3,4-ethylenedioxythiophene) (PEDOT). The resultant biosensors were characterized in terms of their maximum reaction rates, Michaelis–Menten constants (Km), temperature and pH stabilities. All the copolymer matrices represented higher reaction rates and higher Km values in comparison to both polypyrrole and PEDOT matrices and a relation between the morphology of the matrice and the kinetic parameters was observed. Biosensors maintained their activity even at temperatures as high as 80°C and could be used at pHs higher than 8 with high precision. The amount of phenolics in actual samples (red wines) was investigated using electrodes, and results were compared with those found from Folin–Ciocalteau method. Henc... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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