Four agglutination assays evaluated for measurement of von Willebrand factor (ristocetin cofactor activity)
| Autor: | A. A. M. Ermens, P. J. De Wild, F. van der Graaf, Huib L. Vader |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities medicine.medical_specialty Chromatography Plasma samples biology Chemistry Biochemistry (medical) Clinical Biochemistry Von Willebrand factor ristocetin cofactor medicine.disease Agglutination (biology) chemistry.chemical_compound Endocrinology Von Willebrand factor hemic and lymphatic diseases Internal medicine Blood plasma medicine biology.protein Von Willebrand disease Platelet Ristocetin circulatory and respiratory physiology |
| Zdroj: | Clinical Chemistry. 41:510-514 |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | The concentration of von Willebrand factor (vWf) in patients' plasma can be determined by measuring the ristocetin cofactor activity (vWf R:Co). However, this vWf R:Co assay is time consuming, which limits its routine use. Several commercial vWf R:Co tests, based on agglutination of lyophilized fixed platelets, are available. We evaluated the slide tests and aggregometer assays from Behring and Organon Teknika and compared them with the classic vWf R:Co aggregometer method. The within-run and between-run precisions of the two slide tests were better than those of the aggregometer methods. The correlation studies between the four commercial assays and the classic aggregation method were based on 23 plasma samples (range: 15-450% vWf R:Co). The correlation coefficients, which ranged from 0.923 to 0.950, did not differ significantly (P > 0.1). All four commercial assays gave significantly lower vWf R:Co values than the classic aggregation method (P < 0.01). We conclude that commercially available fixed platelets can be used for the rapid measurement of vWf R:Co with a slide test. The use of the aggregometer is time consuming and may result in a lower precision. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|