Factors involved in early in vitro endotoxin hyporesponsiveness in human endotoxemia
| Autor: | Dasja Pajkrt, A. Nikola Kimmings, Thybout M. Moojen, John K. Meenan, Krista Zaaijer, Jan Wouter ten Cate, Sander J. H. van Deventer |
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| Rok vydání: | 1996 |
| Předmět: |
0301 basic medicine
business.industry 030106 microbiology Immunology Endotoxin Hyporesponsiveness Cell Biology Plasma levels medicine.disease Microbiology In vitro Sepsis 03 medical and health sciences 0302 clinical medicine Infectious Diseases TNF production In vivo medicine Tumor necrosis factor alpha business Molecular Biology 030215 immunology Whole blood |
| Zdroj: | Journal of Endotoxin Research. 3:283-289 |
| ISSN: | 0968-0519 |
| Popis: | Sepsis and endotoxemia induce a state of early tolerance to the biological effects of endotoxin. Relatively little is known about the mechanisms or mediators involved in this endotoxin hyporesponsiveness. In this study endotoxin-induced TNF production of whole blood obtained at different time points from endotoxin challenged volunteers, and endotoxin- and PHA-induced TNF production by healthy PBMNCs in the presence of serum from endotoxin challenged volunteers were compared. Endotoxin-induced TNF production by whole blood was found to be significantly reduced at 2 h after in vivo endotoxin administration, returning to baseline levels at t = 6 h. In addition, a reduction of TNF produced by healthy PBMNCs in the presence of post-endotoxin serum was observed. No correlation was found with the concentration of any of the well described anti-inflammatory mediators investigated nor with plasma levels of endotoxin-binding proteins. |
| Databáze: | OpenAIRE |
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