Benzo[A]Pyrene and Dibenzo[A, L]Pyrene do not Alter gap Junction Communication in Rat Liver Epithelial Cells

Autor:	Garret B. Nelson, Carl F. Blackman, Jeffrey A. Ross, S. G. Benane
Rok vydání:	1999
Předmět:	Polymers and Plastics Organic Chemistry Gap junction Molecular biology Cell activity chemistry.chemical_compound chemistry Biochemistry Benzo(a)pyrene Rat liver DNA adduct Materials Chemistry Pyrene Potency DNA
Zdroj:	Polycyclic Aromatic Compounds. 17:53-62
ISSN:	1563-5333 1040-6638
DOI:	10.1080/10406639908020601
Popis:	Dibenzo[a, l]pyrene (DB[a, l]P) is more potent than benzo[a]pyrene (B[a]P) in causing tumors in rodents. We investigated the possibility that this higher DB[a, l]P potency involves alterations in gap junction communication (GJC), a cell activity associated with cancer promotion processes. Rat liver epithelial cells, treated with various concentrations up to 50 μM (0.0126 mg/ml) B[a]P or 40 μM (0.0121 mg/ml) DB[a, l]P for 24 hours, were examined for changes in GJC; no changes were observed. However, cells treated with the maximum soluble concentrations of these chemicals had between 213 and 378 attomoles of DNA adduct per μg of DNA. Thus the observed lack of GJC alteration is not attributable to an inability of these cells to metabolize PAHs, and indicates that the different potency exhibited by the two chemicals is not associated with alterations in GJC.
Databáze:	OpenAIRE
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