Indirect Immunofluorescence and FISH for Enumerating Contaminated Site Sulfate-Reducers

Autor:	Wendy J. Robertson, Brain J. Mee, P. D. Franzmann
Rok vydání:	2002
Předmět:	medicine.diagnostic_test biology 16S ribosomal RNA biology.organism_classification Microbiology Bioremediation Polyclonal antibodies medicine biology.protein Antibody Sulfate-reducing bacteria Molecular probe Bacteria General Environmental Science Fluorescence in situ hybridization
Zdroj:	Bioremediation Journal. 6:25-38
ISSN:	1547-6529 1088-9868
DOI:	10.1080/10889860290777459
Popis:	A new species of sulfate-reducer, Desulfosporosinus meridiei, was recently isolated from gasoline-contaminated anaerobic groundwater in which degradation of toluene and other hydrocarbons occurred. Ground-water from inside (three sites) and outside (one site) the contaminant plume was probed with specific polyclonal antibodies raised against two strains of D. meridiei (strains T2 and S6). Molecular 16S rRNA probes designed to hybridize with cells of D. meridiei were also used. Cell counts using antistrain T2 antibodies (specific for all strains of D. meridiei and two strains of D. orientis) were similar (103 cells/mL) both inside and outside the plume as were total DAPI counts (106 cells/mL). The numbers of cells stained with antibodies specific for Group B strains of D. meridiei varied between locations. The molecular probes DSP477A and DSP477B were designed for, and were effective on, pure cultures of D. meridiei and were able to distinguish this species from Desulfovibrio desulfuricans in hybridization...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1ea1c7759d72ad25f5179bf8685802db https://doi.org/10.1080/10889860290777459 Zobrazit plný text záznamu Plný text ve formátu PDF