Monitoring toxigenicMicrocystisstrains in the Missisquoi bay, Quebec, by PCR targeting multiple toxic gene loci
| Autor: | Suha Jabaji, Chandra A. Madramootoo, Felexce F. Ngwa |
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| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
biology Health Toxicology and Mutagenesis Cell number General Medicine Microcystin Management Monitoring Policy and Law Toxicology biology.organism_classification Microbiology law.invention Real-time polymerase chain reaction chemistry law Microcystis Genotype Gene Bay Polymerase chain reaction |
| Zdroj: | Environmental Toxicology. 29:440-451 |
| ISSN: | 1520-4081 |
| DOI: | 10.1002/tox.21770 |
| Popis: | The increasing incidence of mixed assemblages of toxic and nontoxic cyanobacterial blooms in Quebec's freshwater bodies over the last decade, coupled with inherent inadequacies of current monitoring approaches, warrants development of sensitive and reliable tools for assessing the toxigenic potential of these water blooms. In this study, we applied three independent polymerase chain reaction (PCR) assays that simultaneously target the microcystin synthetase (mcy) genes A, E, and G to rapidly and reliably detect and quantify potentially toxic Microcystis genotypes in the Missisquoi bay, Quebec, Canada. Linear regressions of quantitative PCR threshold cycles (Ct ) against the logarithm of their respective Microcystis cell number equivalents resulted in highly significant linear curves with coefficients of determination (R(2) ) greater than 0.99 (p 0.90. Thus, PCR-based assays targeting the mcyA, mcyG, and/or mcyE genes can serve as powerful screening tools for rapid and sensitive estimation of microcystin-producing Microcystis genotypes in freshwater water bodies. |
| Databáze: | OpenAIRE |
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